| Reduction of 4-nitrophenol catalyzed by nitroreductase |
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| 作者姓名： | Jie Chen Rong Ji Dai Bin Tong Sheng Yuan Xiao Wei Wei Meng |
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| 作者单位： | Jie Chen~(a，c) Rong Ji Dai~(a，*) Bin Tong~b Sheng Yuan Xiao~a Wei Wei Meng~a a~School of Life Science and Technology，Beijing Institute of Technology，Beijing 100081，China ~bSchool of Materials Science and Engineering，Beijing Institute of Technology，Beijing 100081，China ~cDongfang College，Beijing University of Chinese Medicine，Langfang 065000，China |
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| 摘 要： | The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper.4-Amino- phenol and 4-bydroxylamino-phenol were found in the reductive products.The relationship between reaction time and the reductive ratio were studied.The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions.The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen- insensitive enzyme.The reductive products of 4-nitrophenol were determined by HPLC and MS.
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| 关 键 词： | 4-硝基酚 还原反应 催化 硝基还原酶 生物转化 污染物 |
| 收稿时间： | 2006-04-18 |
Reduction of 4-nitrophenol catalyzed by nitroreductase |
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| Jie Chen Rong Ji Dai Bin Tong Sheng Yuan Xiao Wei Wei Meng.Reduction of 4-nitrophenol catalyzed by nitroreductase[J].Chinese Chemical Letters,2007,18(1):10-12. |
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| Authors: | Jie Chen Rong Ji Dai Bin Tong Sheng Yuan Xiao Wei Wei Meng |
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| Institution: | 1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;2. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China;3. Dongfang College, Beijing University of Chinese Medicine, Langfang 065000, China |
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| Abstract: | The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper.4-Amino- phenol and 4-bydroxylamino-phenol were found in the reductive products.The relationship between reaction time and the reductive ratio were studied.The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions.The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen- insensitive enzyme.The reductive products of 4-nitrophenol were determined by HPLC and MS. |
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| Keywords: | Nitroreductase 4-Nitrophenol Biotransformation Reduction |
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