Capillary zone electrophoresis of aldoses,ketoses and uronic acids derivatized with ethylp-aminobenzoate

Summary A number of mono- and oligosaccharides derivatized with ethylp-aminobenzoate have been separated as their borate complexes by capillary zone electrophoresis, using a fused silica capillary containing 175 mM borate buffer, pH 10.5, as carrier. The derivatized carbohydrates were sufficiently separated within 17 min at an applied voltage of 25 kV. On-column UV monitoring allowed detection of these derivatives at the 10 fmol level, and quantification by the relative peak area method allowed reproducible determination of the saccharides at least in the concentration range of 0.5–20 mM in reaction solutions. This method has been applied to the determination of the monosaccharide compositions of polysaccharides extracted from Flos matricariae and Radix althaeae.