| 毛细管电泳和高效液相色谱对结构相似小分子多肽的分离分析比较 |
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| 引用本文： | 董文玉.毛细管电泳和高效液相色谱对结构相似小分子多肽的分离分析比较[J].分析化学,1997,25(11):1285-1289. |
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| 作者姓名： | 董文玉 |
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| 作者单位： | 军事医学科学院毒物药物研究所 |
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| 摘 要： |
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| 关 键 词： | 高效液相色谱 多肽 CZE MEKC |
Comparison of Capillary Electrophoresis and High-performance Liquid Chromatography in the Analysis of Structurally Related Peptides |
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| Dong Wenyu,Guan Fuyu,Liu Gang.Comparison of Capillary Electrophoresis and High-performance Liquid Chromatography in the Analysis of Structurally Related Peptides[J].Chinese Journal of Analytical Chemistry,1997,25(11):1285-1289. |
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| Authors: | Dong Wenyu Guan Fuyu Liu Gang |
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| Abstract: | To optimize the micellar electrokinetic chromatography (MEKC) method, the buffer composition, buffer pH, the content of organic additive and phosphate concentration are investigated and optimized by a statistic uniform design. The optimal MEKC buffer con- sists of 70 mmol/L of CTMAC, 20% acetonitrile and 70 mmol/L of phosphate at pH 2. 5. By the optimal MEKC method, ten crude peptides are analyzed and the results are compared with that obtained by the optimal CZE and HPLC. In view of the purity, migration time, column efficiency and the total number of peaks in each crude peptide, we might conclude that the MEKC method is superior to HPLC and HPLC superior to CZE. |
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| Keywords: | Micellar electrokinetic chromatography capillary zone electrophoresis high-per- formance liquid chromatography peptide |
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