Different protein expression of myocardium from Chinese mini-swine model of myocardial infarct |
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Authors: | Yanfeng Zhao Nengsheng Ye Rongli Zhang Xue Feng Guoan Luo Yiming Wang |
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Institution: | (1) Analysis Center, Department of Chemistry, Tsinghua University, Beijing, 100084, China |
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Abstract: | High-resolution two-dimensional gel electrophoresis (2-DE), followed by computer-assisted image analysis was used to screen
protein patterns of normal and infarcted myocardial tissues for quantitative and qualitative differences in protein expression.
In the gels of pH 5–8 immobilized pH gradient (IPG) strips, 851 protein spots were detected in normal myocardial tissue and
1 032 protein spots were resolved in infarcted myocardial tissue. Thirteen protein spots only expressed in normal myocardial
tissue, and 14 protein spots only expressed in infarcted myocardial tissue. Results also showed that 49 protein spots displayed
quantitative changes in expression between normal and infarcted myocardial tissue. Eleven protein spots were subjected to
mass spectrometry (MS) analysis and seven proteins were identified by peptide mass fingerprinting (PMF). These proteins may
be involved in cardiovascular injury, and could play an important role in the treatment of coronary heart disease.
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Translated from Chemical Journal of Chinese Universities, 2006, 8(27): 1467–1471 译自: 高等学校化学学报] |
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Keywords: | myocardial infarct myocardial tissue proteome two-dimensional gel electrophoresis |
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