基于铜增强纳米金标记物结合磁分离的溶出伏安免疫分析法用于蛋白质的高灵敏测定

应用铜原位化学放大纳米金颗粒的信号增强特性, 并结合磁分离技术, 提出了一种高灵敏的溶出伏安免疫分析方法. 实验中以人IgG为模式蛋白质, 将抗体修饰的SiO₂@Fe₃O₄核壳型磁性纳米颗粒和纳米金标抗体悬浊液混合, 用以均相免疫识别人IgG, 借助外加磁场分离纯化, 在免疫复合物悬浊液中加入铜增强试剂进行沉积放大反应, 再将铜用稀硝酸溶解并进行溶出伏安分析检测. 结果表明, 与基于固相反应的金属免疫分析法相比, 所提出的基于均相反应和磁分离原理的方法具有操作简单、分析时间短等优点. 该方法显示出明显增强的人IgG检测性能, 其线性检测范围为01~1000 ng/mL, 检出限为73 pg/mL. 此外, 将其用于实际样品的回收率测定, 结果令人满意.

Anodic Stripping Voltammetric Immunoassay Based on Coupling Copper-enhanced Nanogold Tags with Magnetic Separation for Highly Sensitive Detection of Proteins

A novel and highly sensitive anodic stripping voltammetric immunoassay was proposed on the basis of chemical copper-enhanced gold nanoparticle(nanogold) tags in vitro as the amplification approach with the aid of magnetic separation technique.In a homogeneous bulk solution phase,the human IgG(hIgG) as a model of test system was captured by goat anti-human IgG antibody(anti-hIgG)-conjugated SiO2@Fe3O4 magnetic nanoparticle and anti-hIgG-conjugated nanogold,followed by the copper metal dissolution in diluted ...