Synthesis,spectral characterization,electrochemical studies of pesticide and biological evaluation of transition metal complexes of azo dye derived from substituted phenyl pyrazole

Diazotization of p-nitro aniline followed by coupling with 3-methyl-1-phenyl pyrazole to procure biologically active azo-dye ligand 3-methyl-4-[(E)-(4-nitrophenyl) diazenyl]-1-phenyl-1H-pyrazol-5-ol (NDP) and also the array of transition metal complexes of aforementioned azo-dye ligand has been carried off along with the use of metal complex to detect pesticides electrochemically. Structural verification of synthesized compounds was carried out via ¹H and ¹³C NMR along with the percentage of C,H,N, molecular weights, Infra-red, UV-Visible spectra and molar conductance of ligand and its complexes was observed. Also TGA, P-XRD and CV measurements were noted. An antimicrobial potency was evaluated by well diffusion method and antioxidant efficacy by DPPH technique. Molecular docking studies was used and MTT tests were conducted on MCF-7 and Hela cell lines to investigate anticancer potency. Upon utilizing physical and spectroscopic techniques to characterize the novel azo-dye ligand and its transition coordinated compounds justified the bi-dentate nature of ligand. Further CV measurements showed that the synthesized Co(II) complex exhibited excellent electrocatalysis properties for the electrochemical sensing of pesticide(Ammonium salt of glyphosate 71% SG) with wider linear range of 0.1 μM–0.8 μM and detection limit 0.033 μM. Mn(II) and Co(II) complex displayed good anti-microbial activity along with cytotoxic potency whereas Co(II) and Cu(II) compounds indicated good anti-oxidant activity. Thus, NDP and its complexes being structurally supported via spectral data and CV studies affirms that Co(II) is strongly useful in detecting pesticide. And lastly it was found that all metal complexes have admirable bioefficacy when compared to ligand.