Studies on mass production of transformed Panax ginseng hairy roots in bioreactor |
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| Authors: | Jeong Gwi-Taek Park Don-Hee Hwang Baik Park Kyungmoon Kim Si-Wouk Woo Je-Chang |
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| Institution: | (1) Faculty of Chemical Engineering, Chonnam National University, 500-757 Kwangju, Korea;(2) Institute of Bioindustrial Technology, Chonnam National University, 500-757 Kwangju, Korea;(3) Department of Biological Sciences, Chonnam National University, 500-757 Kwangju, Korea;(4) Department of Environmental Engineering, Chosun University, 501-759 Kwangju, Korea;(5) Department of Biology, Mokpo National University, 534-729 Chonnam, Korea |
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| Abstract: | The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly
exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased
to 6.4. After 45 d, hairy roots grew about 16-folds. The growth rate of hairy roots in air-bubble column or stirred bioreactor
cultures was 1.13 (1.11) to 1.23 (1.20) g fresh wt (dry wt)/(g of cells·d), respectively. For both bioreactors, growth was
about three times as high as in the flask cultivation. |
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| Keywords: | Panax ginseng transformed hairy roots ginseng crude saponin polysaccharide Agrobacterium rhizogenes |
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