Bromocresol green as a new spectrophotometric probe for serum albumins |
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Authors: | Meenakshi Maruthamuthu and S Kishore |
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Institution: | (1) Department of Physical Chemistry, University of Madras, Guindy Campus, 600 025 Madras, India |
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Abstract: | Bromocresol green (BCG) has been employed as a new spectrophotometric probe to characterise the binding regions of human serum
albumin (HSA) and bovine serum albumin (BSA). BCG binds with greater affinity onto BSA than onto HSA. Based on the abilities
of ligands Naproxen and l-anilino-8-naphthalenesulphonic acid (ANS) to displace BCG from the serum albumins by competitive
or non-competitive mechanism, binding regions were identified for these ligands. It has been found that both Naproxen and
ANS share common binding sites with BCG in HSA with the relative ability of Naproxen > ANS on binding to HSA. In the case
of BSA, ANS competes with BCG for the same binding sites, whereas Naproxen exhibits non-competitive binding. The highaffinity
sites of Naproxen coincide with BCG binding sites while the low-affinity sites occur at sites distinct from the BCG binding
region. |
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Keywords: | Human serum albumin bovine serum albumin bromocresol green binding region spectrophotometric probe equilibrium dialysis competitive displacement noncompetitive mechanism |
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