In Vitro Interaction of Nicotine and Hemoglobin under Liver Cell Metabolizing Condition

The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing system was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increasing of nicotine dose. Meanwhile, the CD spectra intensity reduced, showing the conformation of Hb varied markedly through the interaction. All these results suggested that the interaction of nicotine or its metabolites and Hb might do harm to physicological function of Hb.