灵芝菌丝体多糖的化学组成和溶液性质

用磷酸盐缓冲液在80℃时从灵芝菌丝体中提取出多糖-蛋白质缀合物LM-A.成分分析表明,GLM-A主要由葡萄糖组成,蛋白质含量为13%.GLM-A在1∶1水稀释的饱和镉乙二胺溶液中30℃时的特性粘数η]和均方根旋转半径〈S²〉^1/2的分子量依赖关系分别为η]=5.1×10^-2M_w^0.06(cm³·g^-1)和〈S²〉^1/2=3.9×10^-2M_w^0.50(nm).按照Yamakawa-Fujii-Yoshizaki蠕虫状圆筒模型的粘度理论和Bohdanecky表达式,求得GLM-A的分子参数为:单位围长摩尔质量M_L=(530±10)nm^-1,持久长度q=(2.8±0.2)nm.链直径d=0.75nm.实验结果表明,GLM-A在该溶液中为无规线团构象.

Chemical Components and Solution Properties of a Glycan-Protein Complex from Ganoderma Lucidum Mycelium

A glycan-protein complex (GLM-A) was isolated from the Ganoderma lucidum with phosphate buffer solution at 80 ℃. The carbohydrate in GLM-Ais almost constituted by glucose, and the content of protein is about 13%. The molecular weight M_w dependences of intrinsic viscosity η] and root mean square radius of gyration 〈 S²〉^1/2 of GLM Ain the 1∶1 water diluted cadoxen at 30 ℃ are η ]=5.1×10^-2 M_w^0.60 (cm³·g^-1 ) and 〈 S²〉^1/2=3.9×10^-2M_w^0.50(nm), respectively. According to Bohdanecky's method based on Yamakawa Fujii Yoshizakis theory for worm like chain cylinder, molar mass per unit contour length M L, persistence length q , and chain diameter d were estimated to be (530±10) nm^-1 , (2.8±0.2) nm, and 0.75 nm, respectively, in the 1∶1 water-diluted cadoxen. The results indicated that the glycan protein complex exists as a random coil in the aqueous solution.