Ag@SiO2纳米复合物金属增强荧光释放法检测葡萄糖

制备了Ag@SiO2纳米复合物，罗丹明B通过物理掺杂结合在SiO2壳层。由于金属增强荧光效应，罗
丹明B的荧光增强到4.7倍。Ag核易被H2O2氧化，Ag核氧化后产生荧光增强释放效应。基于金属增强荧光
释放建立了一种新型葡萄糖检测方法，采用交联法在罗丹明B掺杂的Ag@SO2纳米复合物的SiO2壳层固定
葡萄糖氧化酶。检测浓度范围为0.2～6.8 mmol/L，检测限可达0.06 mmol/L。由于H2O2氧化Ag核反应迅
速，检测体系对葡萄糖的响应快速。

Ag@SiO2 Nanocomposites Providing Metal-Enhanced Fluorescence Release and Glucose detection

Ag@SiO_2 and rhodamine-doped Ag@SiO_2 nanocomposites were prepared. Rhodamine fluorescence intensity was enhanced by a factor of 4.7 due to metal-enhanced fluorescence. The Ag core was easily oxidized by H_2O_2, enhancing the fluorescence release of rhodamine. Glucose oxidase was immobilized on the layer of SiO_2 shell by chemical cross linked cluster. A novel method to detect glucose was developed based on metal-enhanced fluorescence in a concentration range of 0.2～6.8 mmol/L with a detection limit of 0.06 mmol/L. Speedy detection response was obtained upon the fast oxidation of Ag core by H_2O_2.