牛血清白蛋白与铬天青S作用机理的研究

用三种不同方法相互对照,求出了铬天青S与牛血清白蛋白的结合常数和结合个数,提出了蛋白质等电点前后或不同铬天青S浓度下,两者存在不同的结合模式。利用Forster非辐射能量转移理论确定了铬天青S在牛血清白蛋白上的结合位置,利用铬天青S对BSA的荧光猝灭,对两者的作用机理作了初步探讨。

A study on the reaction mechanism between chrome-azurol S and bovine serum albumin

In this paper, three different methods were used to study the binding numbers and binding constants of chrome - azurol S (CAS) on bovine serum albumin (BSA), and almost same results were obtained. It is proposed that the concentration of CAS and pH of the solutions affect the binding mode of CAS and BSA. According to the theory of Forster non - radiative energy transfer, the binding sites of CAS on BSA are found. Taking advantage of fluorescence quenching, the reaction mechanism of CAS and BSA is explored.