| 家蝇幼虫抗菌肽MDL-1的荧光特性分析 |
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| 引用本文： | 宫霞,施用晖,乐国伟. 家蝇幼虫抗菌肽MDL-1的荧光特性分析[J]. 分析科学学报, 2005, 21(4): 426-428 |
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| 作者姓名： | 宫霞 施用晖 乐国伟 |
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| 作者单位： | 江南大学食品学院营养与生物技术研究室,工业生物技术教育部重点实验室,江苏无锡,214036;上海交通大学食品科学与工程系,上海,201101;江南大学食品学院营养与生物技术研究室,工业生物技术教育部重点实验室,江苏无锡,214036 |
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| 基金项目： | 中央级科研院所基础性研究项目 |
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| 摘 要： | 本文研究了家蝇幼虫抗菌肽MDL-1的荧光光谱和淬灭剂对内源性荧光的影响。家蝇幼虫抗菌肽MDL-1在激发波长280 nm时，其荧光光谱为酪氨酸（Tyr）残基和色氨酸（Trp）残基共同提供。结果表明，KI不能淬灭抗菌肽MDL-1的Trp残基的荧光，而丙烯酰胺（Acr）能淬灭几乎所有的Trp残基的荧光（f-0．92）；这说明，Trp残基不是位于抗菌肽分子的表面，而是位于分子的内部。
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| 关 键 词： | 家蝇幼虫抗菌肽 色氨酸残基 荧光光谱 荧光淬灭 |
| 文章编号： | 1006-6144(2005)04-0426-03 |
| 收稿时间： | 2004-01-30 |
| 修稿时间： | 2004-01-30 |
Fluorescent Characterization Analysis of the Antibacterial Peptide MDL-1 from Musua Domestica Larvae |
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| GONG Xia,SHI Yong-hui,LE Guo-wei. Fluorescent Characterization Analysis of the Antibacterial Peptide MDL-1 from Musua Domestica Larvae[J]. Journal of Analytical Science, 2005, 21(4): 426-428 |
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| Authors: | GONG Xia SHI Yong-hui LE Guo-wei |
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| Abstract: | Effects of quenching agents on the intrinsic fluorescence and fluorescence spectroscopy of the antibacterial peptide MDL-1 from Musua Domestica Larvae were studied. The results showed that fluorescence spectra of MDL-1 exhibited characteristic fluorescence of Trp residues and Tyr residuces at 280 nm. The Trp residue fluorescence of MDL-1 was not quenched by KI, but quenched by acrylamide. The fraction of Trp quenched obtained by the modified Stern-Volmer equation was 0. 92. The Trp residues of MDL-1 were located in the inner district with negative dielectric instead of the surface. |
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| Keywords: | Antibacterial peptide Tryptophan(Trp) Fluorescence spectrum Fluorescence quenching |
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