| 富勒醇对四膜虫的紫外防护作用 |
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| 引用本文： | 赵群芬,童丽娟.富勒醇对四膜虫的紫外防护作用[J].宁波大学学报(理工版),2008,21(4):474-478. |
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| 作者姓名： | 赵群芬 童丽娟 |
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| 作者单位： | 宁波大学生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 浙江省自然科学基金
，
宁波大学人才基金
，
宁波大学学科项目
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| 摘 要： | 为探讨富勒醇对紫外光的辐射防护作用，运用四甲基偶氮唑盐微量酶反应比色法（MTT法1检测了紫外辐照后四膜虫细胞的存活率，并测定了经紫外辐照后，富勒醇对细胞内丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽酶（GSH）、谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GSH-PX）的影响．结果显示：富勒醇能提高受紫外辐照后细胞的存活率，并且能通过刺激细胞内抗氧化酶的活性，减少脂质过氧化产物，从而减小紫外辐射对细胞的损伤，说明富勒醇对紫外光辐射有防护作用．
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| 关 键 词： | 富勒醇 四膜虫 紫外防护 |
Radioprotection of Fullerenols for Tetrahymena pyriformis Exposed to Ultraviolet Radiation |
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| ZHAO Qun-fen,TONG Li-juan.Radioprotection of Fullerenols for Tetrahymena pyriformis Exposed to Ultraviolet Radiation[J].Journal of Ningbo University(Natural Science and Engineering Edition),2008,21(4):474-478. |
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| Authors: | ZHAO Qun-fen TONG Li-juan |
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| Institution: | ( Faculty of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China ) |
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| Abstract: | To study the protective effects of fullerenols on Tetrahymena pyriformis cells exposed to ultraviolet radiation and the probable protective mechanisms, the survival rate of the cells is measured using MTT（ methyl thiazolyl tetrazolium） after irradiation. With the conventional methods, also measured are the levels of malondialdehyde （MDA）, superoxide dismutase （SOD）, catalase （CAT）, Guguangantai （GSH）, glutathione reductase （GR）, and glutathione peroxidase （GSH-PX） for Tetrahymena pyriformis. The results show that fullerenols can act to enhance the survival rate for the Tetrahymena pyriformis exposed to ultraviolet radiation. The protection of the damaged fullerenols induced by ultraviolet radiation seems to be effective through stimulating the activity of enzymic antioxidant, and reducing the production of the lipid surpoxidationd. |
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| Keywords: | fullerenols tetrahymenapyriformis ultraviolet radioprotection |
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