基于FTIR,FT-Raman的脱毒繁育和硫磺熏制祁菊花的药用成分分析研究和光谱表征

采用傅里叶变换红外(FTIR)和拉曼(FT-Raman)光谱技术直接、快速地测定了脱毒繁育和硫磺熏制的三组不同祁菊花样品有效药用成分及含量的变化。对所测样品的FTIR,FT-Raman光谱进行分析对比,结果表明,三组祁菊花样品的FTIR光谱在1 800~500cm-1区间多个红外吸收峰的强度存在明显差异,特征峰形状也略有差异。FT-Raman谱特征峰的形状有明显差异。FTIR和FT-Raman谱直观地反映出茎尖脱毒繁育会使祁菊花的挥发油、黄酮类物质等药用成分含量升高,硫磺熏制使其减少。FTIR和FT-Raman光谱技术为检测脱毒繁育及硫磺熏制引起祁菊花有效药用成分的变化建立科学依据,也为检测其物质成分含量提供一种有效方法。

Research on the Medicinal Effective Component and Characterization of Virus-Free Breeding and Sulfur Smoked Qi Chrysanthemum Based on FTIR and FT-Raman

Based on Fourier transform infrared (FTIR)and Raman spectroscopy (FT-Raman),the effective medicinal composi-tion and its content change of Qi chrysanthemum are directly and quickly determined among the virus-free breeding and sulfur smoked samples of three different groups.FTIR and FT-Raman spectra of three groups of Qi chrysanthemum sample are com-pared and analyzed.The results show that the intensities of multiple infrared absorption peaks are obvious different within the range of 1 800~500 cm-1 and the characteristic peak shapes are slightly different in the FTIR spectra of the three groups with obvious differences of characteristic peak shapes in FT-Raman spectrum have.FTIR and FT-Raman spectrum directly reflect that the stem tip virus-free breeding will make the volatile oil,flavonoids and other medicinal component content increase in Qi Chrysanthemum,but the sulfur smoked reduce.The FTIR,FT Raman spectroscopy for detection of effective medicinal compo-sition changes in Qi Chrysanthemum caused by virus-free breeding or sulfur smoked establishes a scientific basis,also an effec-tive method to test their component content.