首页 | 本学科首页   官方微博 | 高级检索  
     


Simultaneous Determination of Phenolics and Polymethoxylated Flavones in Citrus Fruits by Ultra-High Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ-MS)
Authors:Xi Juan Zhao  Donglin Chen  Paul A. Kilmartin  Bi Ning Jiao
Affiliation:1. Laboratory of Quality and Safety Risk Assessment for Citrus Products (Chongqing) Ministry of Agriculture, Citrus Research Institute, College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China;2. School of Chemical Sciences, The University of Auckland, Auckland, New Zealandxijuanzh@swu.edu.cn;4. School of Chemical Sciences, The University of Auckland, Auckland, New Zealand
Abstract:
Phenolic and polymethoxylated flavones are important bioactive components in citrus fruit. Here, a rapid and sensitive method based on ultra-high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) was developed for the simultaneous determination of phenolic and polymethoxylated flavones in the peels and pulp of mandarins, tangelos, and oranges. Three phenolic acids and eight flavonoids, including polymethoxylated flavones, were separated and determined using positive and negative ion modes in a single chromatographic run of only 11?min using the multiple reaction monitoring detection mode. The method was validated with high recoveries from 96.1% to 103.5%, good precision with interday relative standard deviations less than or equal to 7.3%, intraday relative standard deviations ≤2.64%, low limits of detection from 1.0 to 18?µg L–1, and low limits of quantitation in the range from 3.0 to 61?µg L–1. The application of this UHPLC-QqQ-MS/MS method to the citrus extracts of three cultivars showed that mandarin fruits contained the highest total amounts of the 11 analytes, followed by tangelos and oranges. This study provides a reliable and quantitative method that can be used for the development of functional products and quality evaluation of citrus fruits.
Keywords:Mandarins  multiple reaction monitoring (MRM)  oranges  tangelos  ultra-high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS)
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号