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Validated HPLC-Fl Method for the Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine Biomarkers in Human Blood |
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| Authors: | Camelia Albu Simona Carmen Litescu Gabriel Lucian Radu Hassan Y Aboul-Enein |
| Institution: | 1. Centre of Bioanalysis, National Institute for Biological Sciences, Bucharest, 296 Spl. Independentei, Bucharest, Romania 2. Faculty of Applied Chemistry and Material Science, Politechnica University of Bucharest, Bucharest, 1-7 Polizu Str, Bucharest, Romania 3. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, 12311, Egypt |
| Abstract: | The natural methyl donor group, S-adenosylmethionine and its product, S-adenosylhomocysteine play an important role in many biochemical reactions involving transmethylation reactions. These compounds can be used as biomarkers in incipient diagnosis of various pathological disorders therefore the validation of a suitable method to routinely analysis of these compounds is very important. In this paper, a high performance liquid chromatrography method for S-adenosylmethionine and S-adenosylhomocysteine measurement as fluorescent 1,N 6-ethanoderivatives from biological samples was validated in terms of selectivity, linearity range of the response (R?>?0.9993), detection limit (9?×?10?9 and 4.4?×?10?9 molL?1), the limit of quantitation (9.7?×?10?9 and 5.7?×?10?9?mol?L?1), precision, trueness and robustness. The method for quantification simultaneous of these compounds is rapid, sensitive and precise and appropriate for clinical analysis. |
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