|
|||||
|
|
Microwave-assisted guanidinylation in solid phase peptide synthesis: comparison of various reagents |
|
| Authors: | Ewa Witkowska Karolina KubikJolanta Krosnicka Karolina GrabowskaAnna Niescioruk Beata WilenskaAleksandra Misicka |
| Institution: | Faculty of Chemistry, Biological and Chemical Research Center, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland |
| Abstract: | The guanidinylation of a peptide chain on a polymeric support under microwave conditions using derivatives of thioureas—S-alkylisothioureas, pyrazole-carboxamidine, and guanidine as guanidinylating reagents is described. The best results are obtained with N,N′-di-Z-S-methylisothiourea and N,N′-di-Z(2-Cl)-S-methylisothiourea. It is found that guanidinylation with reagents containing Boc groups is accompanied by side reactions. |
| Keywords: | Boc tert-butyloxycarbonyl Dab 2 4-diaminobutyric acid Dap 2 3-diaminopropionic acid Dap(g) 3-guanidino-2-aminopropionic acid DIC 1 3-diisopropylcarbodiimide DIEA diisopropylethylamine hArg(g) 2 6-diguanidinohexanoic acid MW microwave TBTU 2(1H-benzotriazole-1-yl)-1 1 3 3-tetramethyluronium tetrafluoroborate TFA trifluoroacetic acid TIS triisopropylsilane Z benzyloxycarbonyl Z(2-Cl) 2-chlorobenzyloxycabonyl |
| 本文献已被 ScienceDirect 等数据库收录！ | |