| 高效液相色谱法同时测定化妆品中9种激素 |
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| 引用本文： | 鹿毅,杨涛,张煌涛,靳智. 高效液相色谱法同时测定化妆品中9种激素[J]. 光谱实验室, 2010, 27(1): 224-229 |
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| 作者姓名： | 鹿毅 杨涛 张煌涛 靳智 |
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| 作者单位： | 新疆产品质量监督检验研究院,乌鲁木齐市新华南路167号,830004;新疆产品质量监督检验研究院,乌鲁木齐市新华南路167号,830004;新疆产品质量监督检验研究院,乌鲁木齐市新华南路167号,830004;新疆产品质量监督检验研究院,乌鲁木齐市新华南路167号,830004 |
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| 摘 要： | 建立了化妆品中9种激素的HPLC测定方法。样品经甲醇超声提取后,经ZORBAXExtend-C18柱分离,以甲醇-水(pH=8.0,0.02mol/L磷酸盐缓冲体系)为流动相,采用紫外检测器检测。9种激素分离良好并排除了样品中杂质峰的干扰,各激素在1—100mg/L范围内呈良好线性关系(r0.999),平均回收率在93.3%—107.2%之间,相对标准偏差为0.79%—2.07%(n=7),检出限为0.05—0.2mg/L。该方法可简便、快捷、准确的同时测定化妆品中9种激素。
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| 关 键 词： | 激素 化妆品 高效液相色谱法 |
Simultaneous Determination of Nine Hormones in Cosmetics by HPLC |
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| LU Yi,YANG Tao,ZHANG Huang-Tao,JIN Zhi. Simultaneous Determination of Nine Hormones in Cosmetics by HPLC[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(1): 224-229 |
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| Authors: | LU Yi YANG Tao ZHANG Huang-Tao JIN Zhi |
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| Affiliation: | Institute of Xinjiang Production Quality Inspection;Urumqi 830004;P.R.China |
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| Abstract: | An efficient method for analyzing nine hormones by high performance liquid chromatography was developed.Samples were extracted with methanol by ultrasonic,separated through ZORBAX Extend-C18 column and determined with UV detector.Methanol-water(pH=8.0)was used as mobile phase.Each hormone was separated nicer and obviated impurity disturbing.The linear relationship exceeded 0.999 in the range of 1-100mg/L.The average recoveries for the nine hormones were from 93.3%to 107.2%with relative standard deviations of 0.79%-2.07%(n=7),and the limits of detection were from 0.05 to 0.2mg/L.The method is suitable to simultaneous determine nine hormones in cosmetics with simplicity and accuracy. |
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| Keywords: | Hormones Cosmetics High Performance Liquid Chromatography |
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