Discovery of new mutually orthogonal bioorthogonal cycloaddition pairs through computational screening
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| Authors: | Maruthi Kumar Narayanam Yong Liang K N Houk Jennifer M Murphy |
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| Institution: | a Crump Institute for Molecular Imaging , David Geffen School of Medicine , University of California , Los Angeles , California 90095 , USA . Email: ;b Department of Chemistry and Biochemistry , University of California , Los Angeles , California 90095 , USA . Email: ;c Department of Chemical and Biomolecular Engineering , University of California , Los Angeles , California 90095 , USA |
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| Abstract: | Density functional theory (DFT) calculations and experiments in tandem led to discoveries of new reactivities and selectivities involving bioorthogonal sydnone cycloadditions. Dibenzocyclooctyne derivatives (DIBAC and BARAC) were identified to be especially reactive dipolarophiles, which undergo the (3 + 2) cycloadditions with N-phenyl sydnone with the rate constant of up to 1.46 M–1 s–1. Most significantly, the sydnone-dibenzocyclooctyne and norbornene-tetrazine cycloadditions were predicted to be mutually orthogonal. This was validated experimentally and used for highly selective fluorescence labeling of two proteins simultaneously. |
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