An improved HPLC system for the analysis of photosynthetic pigments |
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| Authors: | G W Francis B Huseby Ø M Andersen |
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| Institution: | (1) Department of Chemistry, University of Bergen, Allégaten 41, N-5007 Bergen, Norway |
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| Abstract: | Summary A high performance liquid chromatography system for the analysis of photosynthetic pigments is presented. The method employs an octadecylsilica stationary phase, a programmed quaternary mobile phase consisting of mixtures of methanol, acetonitrile, water and hexane, and a photodiode array detector. Carotenoids and chlorophylls are rapidly analysed in a single chromatographic separation. Thecis-trans isomers of most carotenoids are separated by this method. |
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| Keywords: | Column liquid chromatography Carotenoid pigments Chlorophylls Photosynthestic tissue |
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