| 催化动力学荧光法测定洋葱中的槲皮素 |
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| 引用本文： | 杨慧仙,向艳,于军晖,程健琳,邓屹杉.催化动力学荧光法测定洋葱中的槲皮素[J].分析测试学报,2010,29(12). |
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| 作者姓名： | 杨慧仙 向艳 于军晖 程健琳 邓屹杉 |
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| 作者单位： | 南华大学公共卫生学院; |
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| 摘 要： | 研究了稀硫酸介质中,槲皮素催化溴酸钾氧化吖啶红的荧光光谱。在优化实验条件下,确定了荧光强度改变值(ΔIF)与槲皮素质量浓度的关系,建立了催化动力学荧光法测定洋葱中槲皮素的新方法。该方法的线性范围为0.43~18.0μg.L-1,检出限为0.13μg.L-1。方法操作简便,具有较好的灵敏度,用于洋葱中槲皮素含量的测定,结果满意。
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| 关 键 词： | 槲皮素 催化 荧光法 吖啶红 |
Determination of Quercetin in Onion by Catalytic Kinetic Fluorescence Analysis Method |
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| YANG Hui-xian,XIANG Yan,YU Jun-hui,CHENG Jian-lin,DENG Yi-shan.Determination of Quercetin in Onion by Catalytic Kinetic Fluorescence Analysis Method[J].Journal of Instrumental Analysis,2010,29(12). |
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| Authors: | YANG Hui-xian XIANG Yan YU Jun-hui CHENG Jian-lin DENG Yi-shan |
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| Institution: | YANG Hui-xian,XIANG Yan,YU Jun-hui,CHENG Jian-lin,DENG Yi-shan(School of Public Health,University of South China,Hengyang 421001,China) |
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| Abstract: | In sulphuric acid medium,the fluorescence signal of acridine red could be decreased by the oxidation of potassium bromate.In the presence of quercetin,the oxidation reaction was catalyzed,which could cause the fluorescence intensity of acridine red becoming weaker.And the reduced fluorenscence intensity was in proportion to the concentration of quercetin in a certain range.Based on this,a catalytic kinetic fluorescence method was established to detect quercetin in onion.Under the optimal conditions,the line... |
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| Keywords: | quercetin catalytic fluorescence spectrophotometry acridine red |
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