压电免疫传感器用于B因子的测定

B因子是一种不耐热的球蛋白,它参与机体的防御,在炎症过程、细胞和组织损伤中均起重要作用.B因子的检测方法常用的有单扩散法、火箭电泳法和溶血法,前者灵敏度不高,重复性差;后两者操作较复杂^1,2].

Development of a Piezoelectric Immunosensor for the Detection of Factor B

A highly sensitive piezoelectric immunosensor has been developed for the detection of factor B. The sensor consists of an 9 MHz piezoelectric crystal with an antibody on its gold electrodes surface that selectively interacts with the relative antigen. The method of immobilization of the antibody protein with polyethyleneimine adhesion and glutaraldehyde crosslinking was employed. The immobilization methods gave the good results in terms of sensitivity, stability and reproducibility. The influence factors related to the sensitivity of the system such as the titer of antibody, the reaction time of immunoreaction etc., were discussed. Under the optimized experimental conditions chosen, the coated crystal had a good response to Factor B in the mass concentration range of 10-510-3 g/L. This sensor had a good selectivity, other materials in human serum did not interfere the detection remarkably. The recovery of sensor system is about 82%. The crystal could be regenerated by desorption of the bound antigenwith 02 mol/L ethanolamine(pH=8). The sensor could be reused nearly 6 times without detectable loss of activity.