3'-immobilized probes with 2'-caps: synthesis of oligonucleotides with 2'-N-methyl-2'-(anthraquinone carboxamido)uridine residues |
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| Authors: | Al-Rawi Samy Ahlborn Carolin Richert Clemens |
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| Institution: | Institute for Organic Chemistry, Universit?t Karlsruhe (TH), D-76131 Karlsruhe, Germany. |
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| Abstract: | reaction: see text] A synthesis for oligodeoxynucleotides with a 3'-terminal 2'-N-methyl-2'-acylamido-2'-deoxyuridine residue was developed. Unlike their unmethylated counterparts, these oligodeoxynucleotides can be stably immobilized on aldehyde-displaying glass surfaces to provide DNA microarrays. An anthraquinone carboxamido group as a 2'-substituent doubled the capture efficiency of an immobilized tetradecamer. |
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