Electrochemical immunoassay for subgroup J of avian leukosis viruses using a glassy carbon electrode modified with a film of poly (3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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**Figure**
*Schematic illustration of the stepwise immunosensor fabrication process*
Electrochemical immunoassay for subgroup J of avian leukosis viruses using a glassy carbon electrode modified with a film of poly (3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody

Authors:	Zhenzhen Wang Kun Shang Jing Dong Ziqiang Cheng Shiyun Ai

Institution:	2. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China 1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

Abstract:	We have modified a glassy carbon electrode (GCE) with a film of poly(3-thiophene boronic acid), gold nanoparticles and graphene, and an antibody (Ab) was immobilized on its surface through the covalent bond formed between the boronic acid group and the glycosyl groups of the Ab. Subgroup J of avian leukosis viruses (ALV-J) were electrochemically determined with the help of this electrode. There is a linear relationship between the electron transfer resistance (R _et) and the concentration of ALV-J in the range from 527 to 3,162 TCID₅₀?mL^?1 (where TCID₅₀ is the 50?% tissue culture infective dose). The detection limit is 210 TCID₅₀?mL^?1 (at an S/N of 3), and the correlation coefficient (R) is 0.9964. The electrochemical immunoassay showed good selectivity, stability and reproducibility.

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