卵清蛋白与离子型表面活性剂的相互作用

本文通过荧光光谱法、紫外-可见吸收光谱法和透射电镜并结合电导率测定分别研究了水中卵清蛋白与阴离子表面活性剂十二烷基硫酸钠（SDS）和阳离子表面活性剂十二烷基三甲基溴化铵（DTAB）和十六烷基三甲基溴化铵（CTAB）之间的相互作用。研究结果表明卵清蛋白可以增加SDS和CTAB的临界胶束浓度，但对DTAB的临界胶束浓度没有影响。阴离子表面活性剂可以使卵清蛋白构象完全伸展，而阳离子表面活性剂却不具备此种作用。表面活性剂单体与卵清蛋白的相互作用强于表面活性剂胶束与卵清蛋白的相互作用。

Interactions of Ovalbumin with Ionic Surfactants

The interactions of ovalbumin (OVA) with one anionic surfactant, sodium dodecyl sulfate (SDS), and two cationic surfactants, dodecyl trimethylammonium bromide (DTAB) and cetyl trimethylammonium bromide (CTAB), in water have been studied through fluorescence and UV‐Vis spectroscopies and transmission electronic microscopy, combined with the measurement of conductivity. OVA can increase the critical micelle concentrations (cmc) of SDS and CTAB but has little effect on that of DTAB. The interaction between surfactant monomer and OVA is greater than that between surfactant micelles and OVA. Moreover, SDS can make OVA unfolded while cationic surfactants cannot.