HPLC with Column Switching Coupled to APCI–MS for Pharmacokinetic Study of Amygdalin in Rabbit Plasma |
| |
| Authors: | Xinfeng Zhao Weijin Zang Xin Zhao Shixiang Wang Xiaohui Zheng Jianbin Zheng |
| |
| Institution: | (1) Department of Pharmacology, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education, School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, People’s Republic of China;(2) Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Elecetroanalytical Chemistry, Northwest University, Xi’an, 710069, People’s Republic of China |
| |
| Abstract: | A fast and validated assay was established for the pharmacokinetic study of amygdalin in Armeniacae Semen in rabbit. The method involved column switching (CS) enrichment, separation, post-column derivatization, and atmospheric pressure
chemical ionization (APCI) mass spectrometric detection. Plasma sample was enriched by CS using a MAYI-ODS as the first column.
Analytes of interest were isolated and analyzed on a second column of Zorbax SB-C18. To detect amygdalin in plasma samples, a T-piece was connected between the HPLC outlet and the APCI source to add a mixture
of dichloromethane and methanol to the eluent by an isocratic pump. Calibration graphs showed good linearity over a range
of 1.0–1,280 ng mL−1. The detection limit was 0.2 ng mL−1. The intra- and inter-day accuracies were within 3.9%. The method was successfully applied to a study of the pharmacokinetics
of amygdalin after an intravenous injection of amygdalin extracts to rabbits with a dose of 400 mg kg−1. The results indicate that amygdalin is a one-compartment open model with a first order absorption phase. |
| |
| Keywords: | Column liquid chromatography Column switching APCI– MS detection Pharmacokinetics Amygdalin |
| 本文献已被 SpringerLink 等数据库收录！ |
|
