| 葡萄糖发酵液D-核糖含量的高效液相色谱分析 |
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| 引用本文： | 张津枫,邓国才,杨四海,陈荣悌,王健刚.葡萄糖发酵液D-核糖含量的高效液相色谱分析[J].高等学校化学学报,2001,22(1):43-45. |
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| 作者姓名： | 张津枫 邓国才 杨四海 陈荣悌 王健刚 |
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| 作者单位： | 1. 南开大学化学系,
2. 天津市红十字医院, |
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| 摘 要： | D－核糖在生命科学研究领域十分重要，在医药、食品、饲料等工业部门广泛应用，近年来，D－核糖可通过D－葡萄糖在莽草酸营养缺陷型突变株菌种^1]存在下，经发酵制得，目前国内葡萄糖发酵液中该糖含量分析仍采用苔黑酚光度法，此法没有对发酵液中种种上糖类化合物预先分离，选择性差，得到的是各种糖经合物总含量分析结果，糖类化合物测定的高效液相色谱法有衍生法2] ,但比较繁琐，近年RI检测器应用于糖类分析日益增多3,4]，但用于葡萄糖发酵液中D－核糖分析尚未见报道，本文采用高效液相色谱法分析葡萄糖发酵液D－核糖含量，并获得良好结果。
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| 关 键 词： | 高效液相色谱法 D－核糖 葡萄糖 发酵液 分析 |
| 文章编号： | 0251-0790(2001)01-0043-03 |
High Performance Liquid Chromatography Analysis of D-Ribose Content in Glucose Zymotic Fluid |
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| ZHANG Jin-feng,DENG Guo-cai,YANG Si-Hai,CHEN Rong-Ti,WANG Jian-Gang.High Performance Liquid Chromatography Analysis of D-Ribose Content in Glucose Zymotic Fluid[J].Chemical Research In Chinese Universities,2001,22(1):43-45. |
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| Authors: | ZHANG Jin-feng DENG Guo-cai YANG Si-Hai CHEN Rong-Ti WANG Jian-Gang |
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| Abstract: | The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid.D-ribose was determined by HPLC,on a Sugar Pak Ⅰ column,by using a refractive index detector and a mobile phase of pure water.We have also studied the separation conditions of sugars.It has many advantages,such as rapid analysis,high sensitivity and high precision and good reproducibility.The recovery of D-ribose were 95.5％－104％,RSD were less than 1.5％,the detection limit of D-Ribose was 50 ng. |
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| Keywords: | High performance liquid chromatography D-ribose Glucose |
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