4,4'-二(对氨基苯乙炔基)-6,6'-二[N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶的合成与表征

以4,4'-二硝基-2,2'-联吡啶-6,6'-二亚甲基双三氟乙酸酯作为起始原料, 经水解、 溴化、 酯化和对氨基苯乙炔取代4步反应合成了4,4'-二(对氨基苯乙炔基)-6,6'-二N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶. 通过红外光谱、 核磁共振波谱、 高分辨质谱等表征了该化合物的结构. 该化合物经水解后与铕离子形成稀土荧光螯合物, 在紫外光激发下, 发射出具有铕离子特征的荧光光谱.

Synthesis and Characterization of 4,4′-Di(p-aminophenylethynyl)-6,6′bis[N,N-bis(ethoxycarbonylmethyl)amino methyl]-2,2′-bipyridine

In order to obtain a new solid phase time-resolved fluorescence immunoassay chelate,a new intermediate compound 4,4′-(p-aminophenylethynyl)-6,6′-bisN,N-bis(ethoxycarbonylmethyl)amino methyl]-2,2′-bipyridine was prepared from 4,4′-dinitro-2,2′-bipyridine-6,6′-dimethyl bis(trifluoroacetate) by hydrolyzation,bromination,esterification and substitution.The structure of the compound was charactered by DSC,IR,1H NMR and HRMS.The compound was hydrolyzed in the solution of europium ion,forming a rare earth fluorescence chelate.The fluorescence spectrum of the chelate excited by an light source has peaks peculiar to the europium ion.