Reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment |
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Authors: | Grady R Blacken Michael Volný Matthew Diener Karl E Jackson Pratistha Ranjitkar Dustin J Maly Franti?ek Ture?ek |
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Institution: | 1. Department of Chemistry, University of Washington, Seattle, Washington, USA
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Abstract: | Zirconium, titanium, and hafnium oxide-coated stainless steel surfaces are fabricated by reactive landing of gas-phase ions
produced by electrospray ionization of group IVB metal alkoxides. The surfaces are used for in situ enrichment of phosphopeptides
before analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To evaluate this method we characterized
ZrO2 (zirconia) surfaces by (1) comparison with the other group IVB metal oxides of TiO2 (titania) and HfO2 (hafnia), (2) morphological characterization by SEM image analysis, and (3) dependence of phosphopeptide enrichment on the
metal oxide layer thickness. Furthermore, we evaluated the necessity of the reactive landing process for the construction
of useful metal oxide surfaces by preparing surfaces by electrospray deposition of Zr, Ti, and Hf alkoxides directly onto
polished metal surfaces at atmospheric pressure. Although all three metal oxide surfaces evaluated were capable of phosphopeptide
enrichment from complex peptide mixtures, zirconia performed better than hafnia or titania as a result of morphological characteristics
illustrated by the SEM analysis. Metal oxide coatings that were fabricated by atmospheric pressure deposition were still capable
of in situ phosphopeptide enrichment, although with inferior efficiency and surface durability. We show that zirconia surfaces
prepared by reactive landing of gas-phase ions can be a useful tool for high throughput screening of novel phosphorylation
sites and quantitation of phosphorylation kinetics. |
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