Evaluation of methods for the separation of trimethylselenonium ion as a major,minor or general metabolite using neutron activation analysis |
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| Authors: | A J Blotcky J P Claassen E P Rack D M Shoop |
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| Institution: | (1) Department of Chemistry, University of Nebraska-Lincoln, 68588-0304 Lincoln, Nebraska, (USA);(2) Medical Research, Department of Veterans Affairs Medical Center, 68105 Omaha, Nebraska, (USA) |
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| Abstract: | Trinethylselenonium (TMSe) ion was separated by dual-column ion-exchange chromatography and assayed by neutron activation analysis. The TMSe content of the selenium in human urine was found to be (14±2)%, consistent with literature values. An altemate, published, multi-step procedure employing Reineckate precipitation followed by decomposition/volatilization conversion of selenium into dimethylselenide, perchloric acid digestion into selenite and subsequent analysis employing inductively coupled plasma mass spectrometry (ICP-MS) was evaluated. A maximum recovery of TMSe was estimated at 32%. It has been suggested that losses in each step of a multi-step procedure yield low recoveries as reported in the literature. |
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