An improved loopless mounting method for cryocrystallography |
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Authors: | Qi Jian-Xun and Jiang Fan |
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Institution: | Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China |
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Abstract: | Based on a recent loopless mounting method, a simplified
loopless and bufferless crystal mounting method is developed for
macromolecular crystallography. This simplified crystal mounting
system is composed of the following components: a home-made glass
capillary, a brass seat for holding the glass capillary, a flow
regulator, and a vacuum pump for evacuation. Compared with the
currently prevalent loop mounting method, this simplified method has
almost the same mounting procedure and thus is compatible with the
current automated crystal mounting system. The advantages of this
method include higher signal-to-noise ratio, more accurate
measurement, more rapid flash cooling, less x-ray absorption
and thus less radiation damage to the crystal. This method can be
extended to the flash-freeing of a crystal without or with soaking
it in a lower concentration of cryoprotectant, thus it may be the best
option for data collection in the absence of suitable
cryoprotectant. Therefore, it is suggested that this mounting method
should be further improved and extensively applied to
cryocrystallographic experiments. |
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Keywords: | cryoprotectant crystal mounting data collection flash
cooling |
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