Inactivation of Escherichia coli phages by PEF treatment and analysis of inactivation mechanism |
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| Affiliation: | 1. Department of Chemical and Environmental Engineering, Graduate School of Engineering, Gunma University, 1-5-1 Tenjincho, Kiryu, Gunma 376-8515, Japan;2. College of Environmental Science and Engineering, Dalian Maritime University, 1 LingHai Road, Dalian 116023, PR China;1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 360015, PR China;2. Technology Center of China Tobacco Fujian Industrial Co., Ltd, Xiamen, 361022, PR China;3. School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, PR China;1. Hanlong Industrial Co., Ltd, New Taipei City, Taiwan;2. Department of Pediatrics, Landseed Hospital, Taoyuan City, Taiwan;3. Institute of Energy Engineering, National Central University, Taoyuan City, Taiwan;4. Department of Mechanical Engineering, National Central University, Taoyuan City, Taiwan;1. Department of Clinical Pharmacology, Cambridge University Hospital NHS Foundation Trust, Cambridge, United Kingdom;2. Department of Allergy, Guy''s and St Thomas'' NHS Foundation Trust, London, United Kingdom;3. Clinical Immunology and Allergy Unit, Sheffield Teaching Hospital, Northern General Hospital, Sheffield, United Kingdom;4. Faculty of Science, Liverpool Hope University, Liverpool, United Kingdom;5. Department of Allergy, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom;1. Department of Veterinary Pathology and Public Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan;2. Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, 22110, Jordan;1. Department of Orthopaedics, Walter Reed National Military Medical Center, United States;2. Department of Orthopaedics and Podiatry, US Naval Hospital Okinawa, Camp Foster, FPO, United States |
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| Abstract: | Inactivation of phage by pulsed electric field (PEF) treatment using Escherichia coli phages (M13mp18 and λ phage) as the phage particle model was studied. E. coli phages were successfully inactivated, and the absence of degradation of biological molecules was confirmed by electrophoretic analysis of these molecules from the inactivated phages. The comparison of sensitivity to PEF treatment between the E. coli phage and the E. coli cells was also carried out. The E. coli phages were more sensitive to the PEF treatment than the E. coli cells, and this difference allowed the preferential inactivation of E. coli phages. |
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| Keywords: | Pulsed electric field Phage Inactivation |
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