Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins |
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Authors: | Sven O Hagge Malte U Hammer Andre Wiese Ulrich Seydel Thomas Gutsmann |
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Institution: | (1) Department of Immunochemistry and Biochemical Microbiology, Division of Biophysics, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee1-40, D-23845, Borstel, Germany |
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Abstract: | Background The first target of antimicrobial peptides (AMPs) is the bacterial membrane. In the case of Gram-negative bacteria this is
the outer membrane (OM), the lipid composition of which is extremely asymmetric: Whereas the inner leaflet is composed of
a phospholipid mixture, the outer leaflet is made up solely from lipopolysaccharides (LPSs). LPS, therefore, represents the
first target of AMPs. The binding and intercalation of polycationic AMPs is driven by the number and position of negatively
charged groups of the LPS. Also, proteins other than cationic AMPs can interact with LPS, e.g. leading eventually to a neutralization
of the endotoxic effects of LPS. We compared different biophysical techniques to gain insight into the properties of the electrical
surface potentials of lipid monolayers and aggregates composed of LPSs and various phospholipids and their interaction with
peptides and proteins. |
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Keywords: | |
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