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NMR‐based analysis of the chemical composition of Japanese persimmon aqueous extracts
Authors:Shoraku Ryu  Kazuo Furihata  Masanori Koda  Feifei Wei  Takuya Miyakawa  Masaru Tanokura
Abstract:Japanese persimmon (Diospyros kaki L.) is recognized as an outstanding source of biologically active compounds relating to many health benefits. In the present study, NMR spectroscopy provided a comprehensive metabolic overview of Japanese persimmon juice. Detailed signal assignments of Japanese persimmon juice were carried out using various 2D NMR techniques incorporated with broadband water suppression enhanced through T1 effects (BB‐WET) or WET sequences, and 26 components, including minor components, were identified. In addition, most components were quantitatively evaluated by the integration of signals using conventional 1H NMR and BB‐WET NMR. This is the first detailed analysis combined with quantitative characterization of chemical components using NMR for Japanese persimmon. Copyright © 2015 John Wiley & Sons, Ltd.
Keywords:Japanese persimmon  NMR  broadband WET  quantification
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