A New Approach to High Sensitivity Liquid Chromatography-Mass Spectrometry of Peptides using Nanoflow Solvent Assisted Inlet Ionization |
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Authors: | Beixi?Wang Ellen?D?Inutan Email author" target="_blank">Sarah?TrimpinEmail author |
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Institution: | (1) Department of Chemistry, Wayne State University, Detroit, MI 48202, USA; |
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Abstract: | Liquid chromatography (LC) solvent assisted inlet ionization (SAII) mass spectrometry (MS) was previously reported to give
good chromatographic resolution and MS detection injecting 66 ng of a BSA tryptic digest. In analogy to nano-electrospray
ionization (nESI), we extend SAII LC/MS to nano-SAII (nSAII) operating at nL min–1 flow rates and demonstrate good quality ion chromatograms and mass spectra from injection of as little as 0.7 ng of BSA digest
onto a capillary LC column. Data dependent fragmentation is demonstrated for injection of 7 ng of a BSA digest. This method
has advantages over nESI in ease of use and low cost as it requires no voltage and is operational without the necessity of
connectors or fragile nESI emitters, although similar constricted tips can be helpful in nSAII to stabilize the signal at
low nanoliter flow. At a flow rate of 0.8 μL min–1, the only requirement for nSAII is that the exit-end of the capillary LC column be adjusted near the aperture of the heated
inlet of the mass spectrometer. |
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Keywords: | |
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