Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation |
| |
Authors: | K Madhavan Nampoothiri G Jino Tomes Krishnan Roopesh George Szakacs Viviana Nagy Carlos Ricardo Soccol Ashok Pankey |
| |
Institution: | (1) Biotechnology Division, Regional Research Laboratory, CSIR, 695 019 Trivandrum, India;(2) Department of Agricultural Chemical Technology, Technical University of Budapest, Geller ter 4, 1111 Budapest, Hungary;(3) Process Biotechnology Laboratory, Federal University of Parana, 81531-970 Curitiba-PR, Brazil |
| |
Abstract: | Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized
the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, starch,
peptone, and minerals supplemented with 3.75% (w/v) wheat bran particles was found to be the best production medium among
the various combinations tried. Further supplementation of this medium with surfactants such as Tween-20 and Tween-80 considerably
enhanced the enzyme yield. A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2%
(v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm. Herein we present details
of a few of the process parameter optimizations. The phytase enzyme was found to be thermostable, and the optimal temperature
for phytase activity was found to be 55°C. However, 80% of the activity still remained when the temperature was shifted to
70°C. |
| |
Keywords: | Thermoascus aurantiacus thermostable phytase submerged fermentation surfactants phytic acid |
本文献已被 PubMed SpringerLink 等数据库收录! |
|