| MOLECULAR CLONING AND CHARACTERIZATION OF THE cDNA CODING FOR HEPATITIS B VIRUS SURFACE ANTIGEN |
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| 作者姓名： | 何葆光 JOSEPH L. MELNICK ALEEM SIDDIQUI WILLIAM S. ROBINSON SIMON W. LAW EUGENE C. LAI |
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| 作者单位： | Shanghai Institute of Biological Products，Ministry of Public Health，PRC，Department of Virology and Epidemiology，Baylor Colleye of Medicine，USA，Department of Medicine，Stanford University School of Medicine，USA，Department of Medicine，Stanford University School of Medicine，USA，Department of Cell Biology，Baylor College of Medicine，USA，Department of Cell Biology，Baylor College of Medicine，USA |
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| 摘 要： | Hepatitis B virus surface antigen (HBsAg) inRNA has been enriched from a hepatoma cell line (PLC/PRF/5) by specific polysome immunoprecipitation and used for cDNA cloning. A HBsAg cDNA clone was identified by in situ hybridization with a cloned viral probe. It was characterized by restriction endonuclease mapping and DNA sequence analysis. Molecular hybridization of PLC/PRF/5 cellular DNA and RNA to ~(32)P]-labeled HBsAg cDNA revealed the integration of at least six copies of the hepatitis B virus (HBV) DNA into the host genome and expression of three DNA species confining HBsAg-specific sequences. The possible role of HBV in the oncogenesis of primary hepatocellular carcinoma is discussed.
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