Preparative production and separation of 2-acetamido-2-deoxymannopyranoside-containing saccharides using borate-saturated polyolic exclusion gels |
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Authors: | Petrásková Lucie Charvátová Andrea Prikrylová Vera Kristová Veronika Rauvolfová Jana Martínková Ludmila Jiménez-Barbero Jesús Aboitiz Nuria Petrus Ladislav Kren Vladimír |
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Institution: | Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenská 1083, 142 20 Prague 4, Czech Republic. |
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Abstract: | A new separation method based on the combination of exclusion and ion exchange chromatography in borate buffer was developed. It allows semi-preparatory and preparatory separation of isobaric N-acylhexosamines (C-2 epimers) and corresponding methyl glycosides (anomers and tautomers). Three types of polyolic gels were tested for these separations. Ion-exchange HPLC was used as a rapid and reliable method for the quantification of the respective analytes. NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions. |
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