低氧胁迫对真鲷(♀)与黑鲷(♂)杂交子一代体内酶活力的影响

通过分析低氧环境下真鲷(P.major♀)×黑鲷(S.macrocephalus♂)杂交子一代体内酶活性的变化评价其耐低氧能力.将杂交子一代置于溶氧量为(1.83±0.27)mg.L-1的水体中,胁迫0、6、12和24h后,取其肝脏、鳃和肌肉检测乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和总抗氧化能力(T-AOC).结果显示:低氧胁迫后LDH活性在肌肉和肝脏中分别升高25.7%和17.6%;脏中SOD活性升高20.2%,随胁迫时间延长活性下降至初始水平;CAT活性下降44.3%～62.4%;T-AOC活力也呈先升后降趋势.说明杂交子一代具有一定的低氧耐受能力,但长时间低氧胁迫会对其造成氧化损伤.

Hypoxia-induced Metabolic and Antioxidant Enzymatic Activies in the P. major♀× S. macrocephalus♂ F1

To examine hypoxia-induced stress at metabolic and antioxidant enzyme levels,we exposed the P.major♀×S.macrocephalus♂ F1 to（1.83±0.27） mg·L-1 to the dissolved oxygen.After 0,6,12 and 24 h exposure,samples of liver,gill,and muscle tissue are extracted and subsequently analyzed to determine the lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,catalase（CAT）,and total anti-oxidative capability（T-AOC）.Hypoxia facilitates increasing the LDH content in the muscle（25.7%） and liver（17.6%）.The activity of SOD returns to control level after an initial increase（20.2%） in liver.Hypoxia decreases the CAT content in the liver（44.3%～62.4%）.T-AOC activity significantly increases in the liver（33.8%～54.1%） and gill（239%）,but decreases during longer hypoxia exposure.The results show that the hybrid has a certain degree of hypoxia tolerance,but its long hypoxia stress may cause oxidative damage.