| Abstract: | A new reagent system has been reported for the extractive separation and simultaneous spectrophotometric de termination of vanadium (V). The method is based on the formation of a water in soluble blue‐violet V(V) complex with N‐hydroxy‐N‐m‐tolyl‐N′‐phenylbenzamidine (HTPBA), and neutral surfactant Triton X‐100 into chloroform over an acidity range of 1.0–10.0 M acetic acid. The complex shows a broad absorption maximum at 570 nm, when measured against a chloroform blank. The λmax (570 nm) of the complex and that of re agent (313 nm) are well separated, hence the excess of the reagent does not interfere in the spectrophotometric de termination of the metal. The molar absorptivity (?) of the complex is (4.74) × 103 1 mol?1 cm?1. The linearity of the calibration curve is followed between 0.5–12.0 μg mL?1 with slope, intercept and correlation coefficient of 9.16× 10?2, 4.5 × 10?3 and 0.999, respectively. The detection limit of the method is 45 μgl?1. The proposed re agent system provides significantly higher tolerance limit for iron (500 μg mL?1) and also various metalions commonly associated with vanadium did not interfere. The method was applied for the deter mi nation of vanadium content of three samples i.e. Spirogyra, Puccinia and Riccia. |
