Screening of potential antibiotic action of cellulolytic fungi |
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Authors: | Garcia-Kirchner Oscar Segura-Granados Marcela Robledo-Bautista Ignacio Duran-Paramo Enrique |
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Institution: | (1) Departamento de Bioprocesos, Unidad Profesional Interdisciplinaria de Biotecnologia, Instituto Politécnico Nacional (UPIBI/IPN), Av. Acueducto s/n Col. La Laguna, Ticomán, 07340 México, D.F., México |
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Abstract: | Twenty different strains of filamentous fungi were initially selected for evaluation of cellulolytic activity using a single
test in a simple mineral salts culture medium with filter paper as the only carbon source. Those fungi strains that were capable
of completely breaking the filter paper strip within 4–8 d were assayed also for antimicrobial action, using Staphyloccocus a ureus ATCC 6538P according to the so-called agar piece method. We screened three different strains with both capacities: the production
of cellulolytic activity and antibiotic action. The experimental results suggest that the fungi Pinicillium sp. FOPCO1, Aspergillus sp. F0Q001, and Cephalosporium sp. F03800 have both capabilities because they grew rapidly on cellulose as the only carbon source and were able to produce
an area of growth inhibition in S. aureus of approx 2.04, 1.57, and 2.39 cm, respectively, on agar plates using the agar piece method. Subsequently, the antibiotic
production obtained with those cellulolytic strains was evaluated by submerged fermentation at the flask level, in a simple
culture medium containing lactose without biosynthesis precursor, obtaining 3670, 2830, and 4060 antibiotic units/mL, referred
to as penicillin G, whereas for cellulolytic activity, the results were 1.34, 1.81 and 0.57 FPU/mL, respectively. |
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Keywords: | Cellulolytic activity antimicrobial action filamentous fungi bioassays |
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