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The preparation and characterization of poly(o-phenylenediamine)/gold nanoparticles interface for immunoassay by surface plasmon resonance and electrochemistry |
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| Authors: | Wang Qiong Tang Hao Xie Qingji Jia Xueen Zhang Youyu Tan Liang Yao Shouzhuo |
| Institution: | aMinistry of Education Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China bState Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China |
| Abstract: | The poly-o-phenylenediamine (PoPD) nonconducting film and gold nanoparticles (AuNPs) were combined to fabricate AuNPs/PoPD film, which is used as a novel biocompatible interface for the immobilization of antibody and develop a simple and sensitive label-free immunoassay for the detection of the related antigen (human immunoglobulin G (IgG)). Surface plasmon resonance (SPR) and electrochemical methods were used to provide the real-time information about the polymer film growth, assembling of various sizes of gold nanoparticles, anti-human IgG antibody (anti-hIgG) immobilization and the antigen–antibody interaction. The microstructures of the PoPD and AuNPs/PoPD films were characterized by atomic force microscopy (AFM). These results demonstrated that AuNPs were uniformly dispersed on the porous surface of PoPD film, which formed a nano-structure biocompatible AuNPs/PoPD interface. The use of gold nanoparticles and PoPD film could enhance the immunoassay sensitivity and anti-nonspecific property of the resulting immunoassay electrode. Additionally, the reproducibility and preliminary application of anti-hIgG/AuNPs/PoPD/Au electrode for SPR detection of hIgG was also evaluated. |
| Keywords: | Gold nanoparticles Poly-o-phenylenediamine Surface plasmon resonance Electrochemistry Immunoassay |
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