Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin-agarose. |
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| Authors: | H Hagiwara T Nagasawa K M Lodhi M Kozuka T Ito S Hirose |
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| Institution: | Department of Biological Sciences, Tokyo Institute of Technology, Japan. |
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| Abstract: | Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin-agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200 micrograms of pure receptor were obtained. |
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