a Groupe LabEl – Laboratoire MIPS, Université de Haute-Alsace IUT Mulhouse, 61, rue A. Camus, 68093, Mulhouse cedex, France;b OptMec, Photonics Research Institute, AIST, Namiki 1-2, Tsukuba, Ibaraki 305-8564, Japan
Abstract:
We propose a comprehensive treatment of theta microscopy based on dipole emission, which better describes fluorescence emission than the isotropic emission model, as fluorescence emission is often polarized. Formulas describing the point spread function for polarized confocal fluorescence theta microscopy are given. Examples are given and some advantages of polarized theta fluorescence microscopy are presented. To cite this article: O. Haeberlé et al., C. R. Physique 3 (2002) 1445–1450.