Proteomics Analysis of T Lymphocytes Damage Induced by Ionizing Irradiation |
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| Authors: | Shi Ying Zhang Xuan Liu Ning Liang Feng Song Fengrui Liu Zhiqiang Liu Shuying |
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| Institution: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;3. School of Public Health, Jilin University, Changchun, Jilin 130021, China;4. Central Laboratory, The Second Hospital of Jilin University, Changchun, Jilin 130041, China;5. College of Pharmacy, Jilin University, Changchun, Jilin 130021, China |
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| Abstract: | Human T lymphocytes were found to be highly radiosensitive and complex cellular responses including apoptosis could be induced upon exposure to X‐ray irradiation. However, the mechanism of apoptosis associated with irradiation was not clear. In this study, a proteomic method was applied to investigation on alteration of proteome of human T‐lymphocyte cells after irradiation. The Jurkat cells were irradiated with 4 Gy X‐ray and the cell lysates were collected at different times after irradiation (6, 12, 18, 24 and 48 h). The whole proteins were separated and quantified by two‐dimensional fluorescence difference gel electrophoresis, and then the differentially expressed proteins were identified by mass spectrometry. 4 proteins exhibited significant irradiation‐induced difference in abundance, including L‐plastin, bifunctional purine biosynthesis protein, tubulin beta chain, beta‐actin. Differentially expressed proteins were reported to be directly or indirectly involved in the function of human T lymphocyte. Thus, this study might provide clues to identify proteins with biological significance related to irradiation. |
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| Keywords: | T‐lymphocytes irradiation electrophoresis apoptosis proteomics L‐plastin |
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