| pH对氟喹诺酮药物与BSA之间相互作用影响的研究 |
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| 引用本文： | 刘世旺,徐艳霞,夏小环.pH对氟喹诺酮药物与BSA之间相互作用影响的研究[J].分析科学学报,2009,25(5). |
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| 作者姓名： | 刘世旺 徐艳霞 夏小环 |
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| 作者单位： | 黄冈师范学院生命科学与工程学院,湖北黄冈,438000 |
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| 基金项目： | 湖北省自然科学基金，湖北省教育厅科学研究重点项目 |
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| 摘 要： | 采用毛细管区带电泳法,通过测定在不同pH值、不同牛血清白蛋白(BSA)浓度缓冲溶液的条件下药物迁移时间的变化,并分别计算出了pH为6.8、7.4和8.0时培氟沙星、左氧氟沙星、氧氟沙星、环丙沙星等四种氟喹诺酮类药物与BSA相互作用的结合常数.结果表明:pH对结合常数有较大影响,四种药物分子结合常数的最大值均出现在pH=6.8时,并随着pH的增大,结合常数值明显下降.根据实验结果,还对四种氟喹诺酮类药物与BSA之间相互作用的类型、作用位置进行了分析探讨.研究结果对于进一步阐明药用机理并迅速开发出更高效的广谱抗菌药物具有较强的理论意义.
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| 关 键 词： | 氟喹诺酮 牛血清白蛋白 毛细管区带电泳 结合常数 |
Study on the Influence of the pH Value on the Interactions between Fluoroquinolones and Bovine Serum Albumin |
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| LIU Shi-wang,XU Yan-xia,XIA Xiao-huan.Study on the Influence of the pH Value on the Interactions between Fluoroquinolones and Bovine Serum Albumin[J].Journal of Analytical Science,2009,25(5). |
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| Authors: | LIU Shi-wang XU Yan-xia XIA Xiao-huan |
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| Institution: | LIU Shi-wang,XU Yan-xia,XIA Xiao-huan(Life Science and Engineering College,Huanggang Normal University,Huanggang 438000) |
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| Abstract: | The interactions between four fluoroquinolones(pefloxacin,levoxfloxacin,oxfloxacin,and ciprofloxacin) and bovine serum albumin(BSA) were studied by capillary zone electrophoresis.The migration behaviors of the fluoroquinolones were investigated in a series of running buffers containing different concentrations of BSA.The binding constants of the fluoroquinolones and BSA were calculated at pH values of the running buffer of 6.8,7.4 and 8.0,respectively.Results showed that pH value has a great influence upon ... |
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| Keywords: | Fluoroquinolones Bovine serum albumin Capillary zone electrophoresis Binding constants |
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