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Identification of radicals spin-trapped in autoxidized linoleic acids: A high performance liquid chromatography and electron spin resonance study |
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| Authors: | J C Evans K R N Rao S K Jackson C C Rowlands M D Barratt |
| Abstract: | Radicals generated in linoleic acid and deuterated linoleic acid have been trapped by the spin trap 2-methyl-2-nitroso-propane (MNP) and identified by electron spin resonance (ESR) and ENDOR spectroscopy. The formation of two distinct secondary alkyl radical adducts (one conjugated, the other non-conjugated, as shown by their UV absorption spectra) was demonstrated by 11, 11-dideuterio-9-cis-12-cis-linoleic acid using HPLC and ESR spectroscopy. |
| Keywords: | Liquid chromatography HPLC Electron spin resonance ESR Spin traps Autoxidation Linoleic acid |