| 酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测 |
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| 引用本文： | 周艳丽,只金芳.酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测[J].电化学,2006,12(2):214-217. |
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| 作者姓名： | 周艳丽 只金芳 |
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| 作者单位： | 中国科学院理化技术研究所,北京,100080;中国科学院理化技术研究所,北京,100080 |
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| 基金项目： | 国家973(2001CB6106011)项目资助 |
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| 摘 要： | 以硼掺杂金刚石(boron-doped d iamond,BDD)薄膜作基底,利用光化学反应将含有碳碳双键的烯丙胺化合物修饰在BDD表面,形成氨基单分子层,再经过酰胺键的连接使酪氨酸酶固定在氨基化的金刚石表面,从而制得酪氨酸酶修饰的电极.应用循环伏安法研究该电极用于酚类化合物(包括苯酚、对甲基苯酚和对苯二酚等)检测的灵敏度、线性范围及其稳定性.
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| 关 键 词： | 硼掺杂金刚石薄膜 烯丙胺 酪氨酸酶 酚类化合物 |
| 文章编号： | 1006-3471（2006）02-0214-04 |
| 收稿时间： | 2005-12-25 |
| 修稿时间： | 2006-01-22 |
Tyrosinase Immobilizarion on Boron-doped Diamond Electrodes and the Determination of Phenolic Compounds |
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| ZHOU Yan-li,ZHI Jin-fang.Tyrosinase Immobilizarion on Boron-doped Diamond Electrodes and the Determination of Phenolic Compounds[J].Electrochemistry,2006,12(2):214-217. |
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| Authors: | ZHOU Yan-li ZHI Jin-fang |
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| Institution: | Technical Institute of Physics and Chemistry of CAS, Beijing 100080, China |
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| Abstract: | As-grown BDD surfaces were first functionalized by photochemically linking vinyl groups of allylamine,producing covalently linked amine-terminated active BDD surfaces.Then the tyrosinase was immobilized onto the BDD surface through peptide linking.Cyclic voltammograms of phenol,pcresol and hydroquinone were measured using the tyrosinase-modified BDD electrode and amperometric characteristics such as sensitivity,linear response range and stability were studied. |
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| Keywords: | Boron-doped diamond Allylamine Tyrosinase Phenolic compounds |
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