量热法研究线粒体代谢的热力学和动力学行为

线粒体是细胞中极为重要的细胞器，是产生细胞所必需的“富能”物质ATP的重要场所，为细胞活动提供所需化学能．在生命体能量代谢过程中除一部分能量用于合成ATP外，其余则以热的形式释出．用精密热量计测出线粒体代谢过程中的热量输出对了解线粒体的功能和代谢机制具有十分重要的意义．用微量热法研究线粒体体外代谢已有一些报导［‘，’］．本工作用精密热量计和差式扫描量热仪侧定了水稻线粒体体外代谢热谱和DSC曲线，计算了水稻线粒体活性增长速率常数，比较了不同保藏时间的水稻线粒体体外代谢的差异，并初步探讨了水稻线粒体在变…

The Study on the Thermodynamic and Kinetic Behaviour for Mitochondrial Metabolism by Means of Calorimetry

The metabolic thermograms and DSC curves of the mitochondria isolated from rice have been determined by using an LKB 2277 bioactivity monitor and a Dupont 910 DSC & 9900 Computer/Thermal Analyzer System. The thermodynamics and kinetics of the rice mitochondrial metabolism out of the cell have been studied. The metabolic thermograms of the rice mitochondria show different stages. If the rice mitochondria have been laid up for different times, the metabolic thermograms and kinetic behaviours of the rice mitochondria are different. Some thermodynamic values and kinetic parameters, as well as some new experimental thermokinetic equations in the activity increasing stage of the rice mitochondria, have been calculated and established. Furthermore, the thermodynamic and kinetic behaviour of the rice mitochondria in the metabolism out of the cell under changeable and low temperatures have been approached preliminarily.